FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n02_MT_6751.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n02_MT_6751.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21502853
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG244830.11385930973903788No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAC232600.022.0416931
CCGGGAA198900.021.437681
AACGTAT253700.020.0055475
GAACGTA258000.019.7291624
GGAACGT249600.019.381743
GGGAACG280250.018.1486552
CGTATTC272500.017.9615637
ACGTATT304200.016.8506936
TATTCAC319400.016.0506869
CATTGTA239550.015.43861145
GTATTCA330950.015.1401768
GAGTGTA162450.014.373759145
TCGTGTT146050.013.306609145
GAGTGTG211000.013.094132145
TTCGCCG146900.012.93343145
TGTCTTA202050.011.233618145
GGGAAAT266050.010.793621
CCATTGT299700.010.767317145
GCGAGCT184000.010.6316072
GCGGGTT94500.010.2827241
Produced by FastQC (version 0.11.5)