FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n02_MT_6331.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n02_MT_6331.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21018373
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT224540.10683034314787354No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGG94700.026.112858145
ACGTCCG112150.025.800234145
TCGTGTT154700.023.907267145
AACGTAT251800.020.2976655
GGTTAGA191750.017.6317394
GAACGTA318650.017.0396614
GTATTCA308000.016.7370579
CGTATTC305850.016.6174348
TATTCAC304050.016.5734929
CGGGAAC283600.016.2970222
ACGTATT322300.016.0595576
CATTGTA175000.015.871237145
AACCGAG217300.015.7912225
CCAACCG151900.015.5880383
CTAACCA129200.015.4101038
CCATTGT248100.015.404015145
CAACCGA185750.015.0443064
AGAGCGG194100.014.683077145
AGGTTAG159700.014.55114655
GAAACTA144650.014.5142034
Produced by FastQC (version 0.11.5)