FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n02_MT_4340.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n02_MT_4340.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23932957
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC1201870.5021819911346517No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG1064240.4446755158587382No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG680360.2842774505465413No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC638290.26669917971272833No Hit
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA461740.19293061028773No Hit
CACACGTGCTACAATGGCGTATACAGAGGGAAGCGAGAGTGCGAGCTGGA426260.17810586464514186No Hit
GCTACACACGTGCTACAATGGCGTATACAGAGGGAAGCGAGAGTGCGAGC369350.15432693920772098No Hit
CTACACACGTGCTACAATGGCGTATACAGAGGGAAGCGAGAGTGCGAGCT314280.13131682808772857No Hit
GCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATT271400.1134001118206998No Hit
CTGGGCTACACACGTACTACAATGGGAGTTAATAGACGGAAGCAATACCG240660.10055589871322627No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAA657150.050.345711
CATTGTA431300.042.942894145
CCATTGT613250.041.70785145
TGGGCTA162200.041.007372
AACGTAT949350.037.3852466
ACGTATT977100.036.620887
GAACGTA995800.035.8308685
GGGAACG989300.035.234583
CGGGAAC967950.034.8596882
TATTCAC1002550.034.2281849
GGAACGT1032500.033.499654
AGATTAT282050.033.16756145
AGTGTAT92450.032.396122145
GGTTAGA282250.032.3743064
CGTATTC1107200.032.227798
GTATTCA1122900.031.9590199
CTGGGCT223000.031.3179361
GAACCTC285000.026.9063229
GGGTTAG327550.026.8866833
CGAGCAA108450.026.7715515