FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n02_MT_31013.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n02_MT_31013.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26422090
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTT189250.022.262932145
GAGTGTG289450.017.161654145
AGAGCGG187950.013.272668145
GAACGTA110600.012.7645594
TTCGCCG239600.012.560396145
AACGTAT109900.011.7252785
TGTCTTG246450.010.180975145
ACGTATT122800.010.1397026
CGGGAAC181950.09.8833611
CGGGAAT126650.09.3895271
GGGGAAT282750.09.3347741
GGAACGT161650.09.2281543
CCGGGAA193700.08.61000251
GTCTTCG936750.08.554225145
ACTAACC110500.08.4515847
TAGATCT352700.08.450423145
CGGAAGC244550.08.421564145
CGTATTC151450.08.4134928
TGTGTAG541850.08.297643145
GTGTCTT1079400.08.203049145
Produced by FastQC (version 0.11.5)