FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n02_MT_31013.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n02_MT_31013.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20079388
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCCG275800.037.679707145
TCTCGGG149150.032.236347145
TCGTGTT316800.028.888819145
GTACTCT129000.027.6184083
CGGTGGG194500.026.47246145
CTAGTTG139450.025.335878
ATGTGTT322800.025.18417145
CTCTAGT148200.024.912956
AGAGCGG226100.024.889532145
TACTCTA160650.023.2543724
ACTCTAG166450.023.2255525
GTAGATG258700.022.874378145
GAGCGTG138400.022.1122145
GCGTCGG212300.021.246918145
TGTACTC176800.020.980172
TAGTTGT174300.020.4786539
TTGTACT191350.018.49091
TCTAGTT240400.017.2859637
CGCCGTT104450.016.871487145
CCGTATG54200.016.591206145
Produced by FastQC (version 0.11.5)