FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n02_MT_30905.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n02_MT_30905.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22666889
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATTAT310400.016.283318145
TCGTGTT139650.015.422236145
AACGTAT157400.014.580426
CGGGAAC166750.013.9464692
CGTGTAT89200.013.8202505145
GTAGATA210850.013.791218145
GAACGTA168750.013.7714485
GGAACGT162500.013.6776964
CCGGGAA188050.012.8788231
ACGTATT189950.012.2346837
CGTATTC189050.011.872338
GGGAACG202000.011.7941943
TGTTAAT246050.010.757252145
TCTCGGT269000.010.082105145
TCGGTGG282000.010.080192145
AGTGTAT137400.09.974862145
CCGTATA47150.09.535464145
GGGGAAT250850.09.5100951
ATTAGTT203750.09.467076145
TTAGTGT651450.09.45058145
Produced by FastQC (version 0.11.5)