FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n02_MT_30904.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n02_MT_30904.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19612585
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT428840.2186555214419721No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT302320.1541459221209239No Hit
GGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGAT253860.12943729753115155No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA240850.12280380174260556No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA215950.1101078720627597No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT215000.10962348920348847No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG201880.10293390697860583No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC197250.10057317788552605No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT54900.035.535411
TGTACTC56400.034.558992
AGTGTAT43650.033.558197145
GACATAC43800.033.0656249
TCGTGTT119000.032.357807145
TAGCATA75750.031.5453628
AGATTAT164550.030.760193145
ATTAGTT70100.030.72345145
GAGTCTA50050.029.9677472
CTCGGCT61600.029.3156871
TCTAGTT74750.027.218977
AACAGAC55850.027.0953775
GCCAGTA88950.027.0321453
AGTCTAA57300.026.1639983
TACTCTA77050.026.0316964
CGGATGT37150.025.5737191
GGCCAAC168250.025.3025591
CAATTAG40500.024.4993153
AACCGAG184800.024.0568355
GTTACGA63750.023.6253644
Produced by FastQC (version 0.11.5)