FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n02_MT_30903.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n02_MT_30903.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19725045
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA48850.021.4912034
TCGTGTT136300.015.96146145
CTATTAG103950.013.930418
CCGGGAA152850.013.2834231
AACGTAT150850.013.1986435
GATCTAT92950.012.8521695
CGGGAAC175750.012.4603161
GAACGTA170050.012.4326454
GGAACGT162900.011.78136353
GGGAACG195900.010.7622852
ACGTATT192250.010.7327376
GTAGATA211850.010.577358145
CGTGTAT85650.010.329509145
TCTCGGT234700.010.227325145
TAGATCT309400.010.19568145
CGTATTC187800.010.17818
GGGGAAT183200.010.0932761
AGGGTGT574750.09.929855145
GTGTAGA450400.09.805418145
TAGGGTG556550.09.772469145
Produced by FastQC (version 0.11.5)