FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n02_MT_30891.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n02_MT_30891.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19193619
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAT105000.013.813108145
CTCTAGT74500.013.410376
ACTCTAG81900.012.464045
CTAGTTG87700.012.3835058
TCTAGTT100050.011.7949897
GTAGATA229600.011.59164145
TCGTGTT159700.011.579398145
CCGGGAT75700.011.3029721
GTGTGTA150900.010.861003145
CGGGGAT116850.010.1770441
TACGTGT618450.09.697318145
GGGGAAT142800.09.4447681
GGGGGAT103950.09.3473141
CGGGAAT108350.09.3023441
TAGATCT385050.09.171969145
GTACTCT121050.09.0338953
GATCTCG378800.08.997847145
TACTCTA129250.08.9625874
CGATCTA43400.08.8415854
GCGGGAT95150.08.8400711
Produced by FastQC (version 0.11.5)