FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n02_MT_30890.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n02_MT_30890.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19416709
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCCG183650.034.82767145
TCGTGTT202100.026.445267145
GTACTCT110700.024.2049473
TACTCTA119650.022.933734
TGTACTC118800.022.7483272
ATGTGTT191550.021.920132145
TAGTTGT136100.020.6954259
TCTAGTT148800.019.5597087
TTGTACT139200.018.962741
CTCTATT135200.018.344091145
AGAGCGG169300.017.647657145
CTCTAGT172200.016.5229026
GTAGATA199300.015.718939145
GTAGATG178300.014.357213145
ACTCTAG211450.013.62715
AGTGTGG215200.013.580306145
CCGTATA41900.012.2882805145
TCTCGGG176500.012.161685145
TACGGAC108450.012.0830635
AACGTAT99700.011.9090096
Produced by FastQC (version 0.11.5)