FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n02_MT_30887.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n02_MT_30887.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18046677
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAA148450.016.4629921
GGGTTAG96500.016.285233
CGGGAAC162500.015.4722392
GAACGTA171050.014.9843174
GGGAACG178050.014.6020553
AACGTAT173000.014.31244855
TCGTGTT132800.014.252248145
GGTTAGA126650.014.0632834
ACGTATT182550.013.4051717
GGAACGT184350.013.2355974
TAGAACC156300.012.136127
TGGGTTA134250.012.0355982
GAACCTC132150.011.72526559
CGTATTC214200.011.4251968
TATTCAC217500.010.81932459
TTAGAAC199550.010.6665386
GATCTCG214500.010.61557145
CTGGGTT221350.010.5168231
GCCGGAT89000.010.348391
CACACGT110450.010.3084411
Produced by FastQC (version 0.11.5)