FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n02_MT_30886.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n02_MT_30886.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20556794
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT393870.19160088873780612No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT273510.13305090278182483No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA240690.11708537819661957No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC219770.10690869403079098No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT218810.10644169513981606No Hit
CGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTAC208000.10118309304456717No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA47200.034.72222145
AGTGTTA285750.033.244984145
CCGTGTT208400.031.247818145
GTACTCT103900.029.7611393
TCGTGTT161850.028.89935145
TAGTTGT109850.027.947839
TACTCTA122400.026.205024
CGTGTAT160250.025.612942145
TGTACTC127850.025.3303152
TTGTACT126100.024.2089711
GGCTCGT78150.023.6250864
GTAGATA256350.023.309607145
GCGTAAC97100.023.279262
CGCGTAA96450.022.5542471
AGGCGTA75950.022.4983488
CTAACCA171050.022.2653278
ATCCCGA295950.020.8196494
CGTGCGT86550.020.5794838
TACGGAC198550.020.2376925
TCTAGTT170300.019.981477
Produced by FastQC (version 0.11.5)