FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n02_MT_30849.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n02_MT_30849.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31924226
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTAGA180950.016.8058264
CCGGGAA369550.014.4612861
TGATCCT230050.013.7854534
AACGTAT421150.013.7186535
GATCCTG249200.013.3064695
TTTGATC280500.013.3021942
CGGGAAC463250.012.788491
CTGGCTC275150.012.736879
GGGTTAG246150.012.6803633
GGGAACG455000.012.5795362
TATTCAC464100.012.5282639
GAACGTA521200.012.2805294
TAGAACC251850.012.1605537
TTGATCC266100.012.02905753
TCCTGGC260100.011.830517
CCTGGCT249500.011.6371268
ACGTATT517600.011.4979276
GTAGATA315850.011.364935145
CGGAGTA151750.011.1649394
GTATTCA523600.011.1042138
Produced by FastQC (version 0.11.5)