FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n02_MT_30848.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n02_MT_30848.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21933430
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG224660.10242811999764743No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC220650.10059986057812206No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA92450.053.182574
ATCTATT124700.041.28426
TCGATCT118950.040.188323
CTCGATC125600.037.6760032
CTATTAG162750.036.4834028
GATCCTG207400.026.8125515
TATTAGT227750.026.262269
CTGGCTC224250.023.5722399
CTACTCT80550.022.833829
GATCTAT233450.022.4248775
GTGTCCT79700.022.1685854
TGATCCT235300.020.4955124
TCCTGGC276000.020.0435167
TCTATTA272550.019.5267897
GATGCCT229900.019.0546995
TCGTGTT101450.018.655819145
GGGAACG462950.018.377032
GGTGGAT186000.018.3267361
TGTCCTA96950.018.2980145
CGGGAAC462250.018.2788641
Produced by FastQC (version 0.11.5)