FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n02_MT_30786.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n02_MT_30786.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18066432
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA144750.025.198236145
GTAGATA209850.022.737236145
TCGTGTT132200.020.679054145
TGTCTTA172350.019.816656145
CTCGGCT82600.017.732371
TTCGCCG194950.017.705347145
CTAACCA98400.016.9250148
CCGTATA49700.016.924755145
AACGTAT191400.016.645115
GCTCAGA116750.016.5588555
GGCATAG187850.016.2658635
GATCCTG105600.016.0445615
GCATAGT192150.015.7510026
GAGTGTG260000.015.67414145
ATAGTTC195850.015.6021788
GAACGTA212650.015.3569114
TAGTTCA200450.015.3167199
CGTGCGT78850.014.8768058
GGTTAGA152550.014.809324
CGGGAAC220800.014.7449321
Produced by FastQC (version 0.11.5)