FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n02_MT_30785.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n02_MT_30785.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22518682
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTT131150.023.333736145
AGAGCGG102450.013.377962145
CGTGTAT74750.012.22361145
CGGGGTT161550.012.0276041
TACGTGT543450.010.635034145
CGGGAAT83700.010.5678251
CGGGAAC89800.010.0921781
AGTACGG69050.010.082024145
ACGTGTT96050.010.041404145
CGGGCAT102600.010.0344191
GTACGTG575550.09.878094145
CAGTACG658300.09.748988145
GGGGAAT213750.09.73481
CCGGGAT72000.09.364871
AGTACGT615300.09.3578145
CGGGGAT92600.09.2389431
TCTCGGT252900.09.204418145
TCGGTGG260150.09.059405145
GTGGTCG200350.08.867821145
ACGTGTA507800.08.825431145
Produced by FastQC (version 0.11.5)