FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31384495
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1118500.35638617094205277No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAC162100.018.267971
GAACGTA176600.018.2237834
CCGGGAA169200.017.4586141
GGAACGT170750.017.1154654
TATTCAC206400.015.2321699
GTATTCA213400.015.104219
GGGAACG236250.013.7496512
GAGCACC130350.013.246455145
CCATTGT155500.013.055356145
CAGTCTA86400.011.93110225-29
GGTTAGA129700.011.6835214
TGGGCTA84300.011.4743532
GATCCCG115400.011.1281613
CGAGGGA82450.010.7583518
AACCGAG104400.010.6444945
GGGCTAC101550.010.5856023
TCCGATC87650.010.41587355-59
TGTACTC70550.010.2318462
GGGAAAT120750.010.1932291
GTACTCT74850.010.120613
Produced by FastQC (version 0.11.5)