FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n01_MT_neg.11.4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n01_MT_neg.11.4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10727590
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1859771.7336326239164621TruSeq Adapter, Index 2 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA306060.28530173133014963TruSeq Adapter, Index 2 (97% over 37bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT161910.15092858694264044No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTATG166800.042.984145
CGTCTTG78900.040.24424145
GGATATC50700.035.1766132
TAGTTGT121450.034.204569
TCTAGTT129150.032.614347
GATATCT55250.031.3607483
TTGTACT137400.029.3898491
CTAGTTG143850.028.9790978
TGCCGTG97300.028.386951145
TGTACTC146700.027.9713332
ATATCTT48600.026.8512864
TACTCTA161750.026.847974
GTACTCT158300.026.7456233
ACTCTAG179950.026.2682235
CTCTAGT188600.024.9868036
TATCTTG53450.024.822095
CTTCTGG107200.023.533745145
CGGATAT63400.021.0405671
TCTCGTT130700.020.300753145
GTAGTCA41250.019.8600988
Produced by FastQC (version 0.11.5)