FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n01_MT_9109.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n01_MT_9109.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17056602
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT394910.23152911699528428TruSeq Adapter, Index 2 (97% over 37bp)
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT331630.19442911313754052No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC297350.17433132343710664No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA249390.14621317892039692No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT236340.13856218254960748No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG204560.11993010096618306No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA202600.11878098580244763No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC199030.11668795461135813No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT194540.11405554283320911No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG184340.10807545371581047No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT172660.1012276653931422No Hit
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG172260.10099315209442068No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTATG122550.037.445152145
GAAACTA131050.033.1932684
CTCGGCT59350.030.1723751
CTAACCA145200.029.408088
GAGCACC104900.029.301888145
AAGAAAC164300.026.4313892
AGAAACT166700.025.9203823
AACGTAT236100.025.6713475
GAACGTA246700.025.0089934
CGCTCAG54650.024.542424
TACTTCC55200.024.0353455
TGCCGTG68700.023.848274145
GCTCAGA70150.023.770515
GTATTCA257200.023.2823648
TATTCAC260950.023.0588429
CTTTTAC59450.023.0485291
CGCTAGG12300.022.987694
TGTACTC35600.022.8086072
CGAGGGA133950.022.7313168
CTCATTT63800.022.498302145
Produced by FastQC (version 0.11.5)