FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n01_MT_8404.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n01_MT_8404.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20755662
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTATG155100.020.425564145
AACGTAT138800.013.424026
GAGCACC215650.012.639875145
GAACGTA153300.012.6270465
ACGTATT157900.012.0752717
GTATGCC499200.011.47247145
CCGGGAA182750.011.4250281
TATGCCG446100.011.424245145
AAGAGCC207000.011.206863145
TATCTCG718500.010.674888145
GGAACGT178700.010.5886674
TCGTATG577950.010.360822145
TGTCCTA87750.010.3275085
AACCGAG171500.010.2301945
CTCGTAT594900.010.163108145
CGGGAAC230650.010.1524591
CGTATGC545050.010.121683145
CCCGGTT139350.09.9888721
CGTATTC197950.09.9617258
CGAGTCT98150.09.8977328
Produced by FastQC (version 0.11.5)