FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n01_MT_8336.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n01_MT_8336.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22324661
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT577160.258530241511842TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATG372300.029.111181145
GTACTCT150250.027.9371173
TCTAGTT173200.026.454137
TAGTTGT165000.025.527819
TTGTACT163450.025.3706461
TGTACTC170900.024.8160272
TACTCTA186200.023.0497574
ACACGTA55900.022.306337145
TACTCGC107300.022.295944145
AACGTAT159850.019.6838976
TGCCGTG113450.019.36198145
GAACGTA171450.018.7322754
CGTGCGT89400.017.597288
GAGCACC183800.017.591425145
CGTATTC186550.016.9050838
AGAAGCG125050.016.059074
GTTACGA46700.015.989864
ACATTAA182100.015.844727145
AGGCGTA93050.015.816238
CGGGAAC215450.015.4112342
Produced by FastQC (version 0.11.5)