FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n01_MT_7784.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n01_MT_7784.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16757759
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG414620.24741971763646917No Hit
CCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCC393120.23458983984672413No Hit
GTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACG363420.2168667063418205No Hit
CTGGGTTAGAGTGGCCATAACACAAGGGTAGTATCCCAACAGCGTCTCCT357130.21311322116519277No Hit
CGGAAAGACCCCATGGAGCTTTACTGCAGTTTGATATTGAGTGTCTGTAC356890.2129700039247491No Hit
CGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTCGGTCCCTATCCGTCGC342750.20453212150860983No Hit
GGGAAATCTCATCTTGAGGTGGGTTTCACACTTAGATGCTTTCAGCGTTT310820.18547826114458382No Hit
ATGCAGGTTACCCGCGACAGGACGGAAAGACCCCATGGAGCTTTACTGCA273080.16295735008481743No Hit
CTGGAACGCATAGAAGTGAGAATGCCGGTATGAGTAGCGAAAGACAGGTG253840.15147610130925024No Hit
CTGGGCTACACACGTGCTACAATGGCTGGTACAACGAGTCGCAAGCCGGT242750.1448582713237492No Hit
GCAGGTTACCCGCGACAGGACGGAAAGACCCCATGGAGCTTTACTGCAGT230400.1374885508259189No Hit
GCCCGTCAGACACTGTCTCCGATAGGGATCACCTATCTGGGTTAGAGTGG222460.13275044712124098No Hit
CTCGCAGTCAAGCTCCCTTATACCTTTACACTCTGCGAATGATTTCCAAC196180.11706815929265958No Hit
GTCAGACACTGTCTCCGATAGGGATCACCTATCTGGGTTAGAGTGGCCAT183110.10926878707349832No Hit
GTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGG176940.10558691051709242No Hit
CCCCAGTCAAACTGCCCGTCAGACACTGTCTCCGATAGGGATCACCTATC174570.10417264026771121No Hit
GGAAAGACCCCATGGAGCTTTACTGCAGTTTGATATTGAGTGTCTGTACC171360.10225710967677719No Hit
GCCCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATT170100.10150521916444794No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCATC119550.041.782887
CATTGTA187500.038.81865145
GACATAC31800.036.932519
CGCGAGC158450.035.3229031
GGGAAAT162500.033.8625561
CGGATGT20950.032.8753851
AGTCACA42650.031.955587145
TAAGTTA49950.030.1899952
GAAATCT186800.029.147213
CTCATCT170350.028.982278
TAGCATA46600.027.536888
AAATCTC195750.027.4814684
TGACGAT48050.027.3099162
AACAGAC43050.027.2822975
ATCTCAT191000.027.1784026
GTAGCAT47700.027.0539267
GGGTTCA208350.026.6884179
GAGCACC71900.026.114328145
GGATGAC222950.025.884795
GATGACG222200.025.3851556
Produced by FastQC (version 0.11.5)