FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n01_MT_7784.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n01_MT_7784.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19885735
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT362810.1824473674219233No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC348220.17511044977718954No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT243480.12243952763123916No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA225120.1132067786279964No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC224350.11281956638766431No Hit
CCTCCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTAC223960.11262344590230132No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC217070.10915865066088831No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT217040.10914356446970655No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG205830.10350635769811878No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTATG134500.036.759384145
GAAACTA121600.036.1897244
GTCGAAT68600.035.720391
GATCCTG74400.034.9827465
AACGTAT411750.032.7500275
GATCATG43200.032.22185
AAGAAAC136600.032.0027242
GTATTCA427900.031.394658
TCCCTGA111000.031.0888253
TCGATCT26300.030.5976123
TTGAACG73650.030.3179957
GAGCACC111400.030.195272145
GAACGTA460750.030.1795964
TATTCAC456500.029.5546559
AGAAACT149850.029.1729893
CGGGAAC363650.028.5086121
CGTATTC476100.028.2010527
ACGTATT485400.028.0648426
CCTCCCT129000.027.8750441
CTAACCA155800.027.4540928