FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n01_MT_6852.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n01_MT_6852.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35298285
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAA482550.024.1883131
GAACGTA546000.022.8782125
CGGGAAC509800.022.7532182
AACGTAT561050.022.2001846
GGAACGT552150.021.7697684
ACCGAGG112800.021.2099576
CGTATTC598350.020.7549488
CATTGTA383250.020.16412145
ACGTATT630900.019.8450987
GTATTCA689900.018.158289
GATCATG210950.017.9742835
TTGAACG280750.017.7918427
TATTCAC677800.017.6374669
CCAACCG137350.017.6295193
CAACCGA205800.017.4728074
GGGAACG727100.017.3391633
CCATTGT521500.017.112284145
CCCCCTT235850.016.8140951
TAACCAG317600.016.6860689
TACGGAC236050.016.677285
Produced by FastQC (version 0.11.5)