FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n01_MT_6751.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n01_MT_6751.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21502853
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG237660.11052486849070679No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAA189450.021.8504121
CGGGAAC220450.019.0737481
GAACGTA244050.017.7644444
GGAACGT236700.017.6416573
AACGTAT244550.017.6393095
CGTATTC266450.016.0802928
GGGAACG270000.015.8149073
ACGTATT302750.014.3921486
TATTCAC308400.014.1750359
CATTGTA239100.013.461932145
GTATTCA326450.013.3024419
CTCGATG247850.013.191423145
GCGAGCT184600.012.6063832
GGGAAAT242250.012.5990111
CGCGAGC211050.011.5074111
ACACGTA69400.010.863703145
CCATTGT309000.010.463582145
GTATGCC389700.09.896583145
GGGTTCA260850.09.6996439
CGTATGC419950.09.684324145
Produced by FastQC (version 0.11.5)