FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n01_MT_6331.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n01_MT_6331.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21018373
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT221880.10556478372517226No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAT251500.019.7176656
CCAACCG136800.016.8524463
GATCCTG145000.016.599865
GAACGTA313600.016.3909655
AACCGAG206800.016.1265545
CAACCGA174450.016.041674
GTATTCA315550.016.0134339
GGTTAGA180350.015.9188734
CTAACCA130350.015.7397688
CGTATTC321700.015.6848778
ACGTATT332100.015.4775197
TATTCAC311800.015.3922379
TGATCCT150000.015.32154
CGGGAAC289050.015.0988322
AGGTTAG164300.014.8705375
GGCCAAC162750.014.7443561
CCATTGT252950.014.501914145
TTGATCC149500.014.3539833
ATCCCGA272000.014.2066474
GAAACTA150250.014.1862024
Produced by FastQC (version 0.11.5)