FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n01_MT_4340.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n01_MT_4340.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23932957
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC1115310.46601429150606No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG1021310.4267379078983011No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG664590.2776882104455375No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC607130.25367947638062444No Hit
CACACGTGCTACAATGGCGTATACAGAGGGAAGCGAGAGTGCGAGCTGGA475760.19878864111944045No Hit
GCTACACACGTGCTACAATGGCGTATACAGAGGGAAGCGAGAGTGCGAGC422180.17640110246301782No Hit
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA385810.16120448467776047No Hit
CGGAAAGACCCCGTGAACCTTTACTATAGCTTGACACTGAACATTGAATT293200.12250889014675453No Hit
CTACACACGTGCTACAATGGCGTATACAGAGGGAAGCGAGAGTGCGAGCT292100.12204927289177013No Hit
GCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATT274010.11449065821661736No Hit
GCGGTGTACCCGCGGCTAGACGGAAAGACCCCGTGAACCTTTACTATAGC269920.11278171769581168No Hit
CTACAATGGCGTATACAGAGGGAAGCGAGAGTGCGAGCTGGAGCGAATCT266920.11152821609130874No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAA627800.047.8312841
CATTGTA430500.040.785698145
TGGGCTA163500.040.660362
CCATTGT616750.038.96559145
AACGTAT921950.035.4886445
ATAGATG150750.034.43203145
ACGTATT952650.034.428347
GAACGTA966600.034.1641964
TATTCAC985500.033.420299
GGGAACG960550.032.9976123
CGGGAAC938800.032.5803762
GGAACGT1003800.031.468064
CTGGGCT225450.030.7740731
CGTATTC1086750.030.266648
CGAGCAA109450.030.0726385
ACGAGCA101500.030.0708474
GTATTCA1113400.029.7765339
TCACGAG108100.028.435432
CTTACAT94700.027.1770061
GGTTAGA268100.026.7441674
Produced by FastQC (version 0.11.5)