FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n01_MT_31013.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n01_MT_31013.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26422090
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGTA107750.011.4382844
AACGTAT106350.011.043736
GAATCGG447950.09.645332145
AATCGGG398450.09.588203145
ACGTATT122950.09.5523997
ATCGGGG387300.09.28399145
CACTCCG992300.09.132033145
GAGCACC242450.09.029947145
GGGGAAT260500.08.9334491
CTCCGGA884500.08.810714145
AGAATCG548100.08.7558565145
TCCGGAG833700.08.6432495145
CGGAGAA780650.08.627145
CCGGGAA188200.08.5517541
AACCGAG120650.08.4727485
TCACTCC1132900.08.408223145
CGGGAAC179550.08.3984621
GTCACTC1133150.08.380777145
GGGAAAT286950.08.3626431
ACTAACC116950.08.1827577
Produced by FastQC (version 0.11.5)