FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n01_MT_31013.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n01_MT_31013.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20079388
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT265550.13225004666476886TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCT130000.018.1245923
CTAGTTG134900.017.5734718
CTCTAGT148300.017.4527976
GAGAATG472900.016.57171145
TACTCTA161900.015.7626194
ACTCTAG171100.015.4659465
ACACGTA66550.015.141807145
TAGTTGT166950.015.0248199
TGTACTC180600.013.4479262
GAGCACC168800.012.884268145
TTGTACT193250.012.1924821
TCTAGTT241400.011.8326447
TAAGCGA45950.011.3601736
TACGACT40850.011.3583742
GTAAGCG46100.011.3230975
CGGGAAT72650.010.6776681
GAACGTA64300.010.0348915
AACGTAT65700.09.9315066
CGGGAAC80850.09.7740541
AAGAGCC205650.09.659014145
Produced by FastQC (version 0.11.5)