FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n01_MT_30979.TR2_batch_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n01_MT_30979.TR2_batch_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14706242
Sequences flagged as poor quality0
Sequence length151
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT398320.27085097606852926No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG388210.26397634419452637No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC371810.2528246169211686No Hit
CCTCCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTAC336380.22873280611049374No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC326480.22200097074425948No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG300180.20411740810466739No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT294800.20045909757230979No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG294190.20004430771641046No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA272980.18562186043178128No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA260240.17695887229381918No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG259730.17661208077495255No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGA233450.158742117802767No Hit
GGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGAT224610.15273106480907903No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT219410.1491951512833802No Hit
AAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGC211630.14390488066223853No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG208170.14155213820090815No Hit
CCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGC205030.13941699041808234No Hit
GCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAA202440.13765583348893617No Hit
CGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGG192500.13089679878788885No Hit
CATGAATGGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAA190950.12984282456388246No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA187650.12759887944180437No Hit
CTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGG181510.12342378154799845No Hit
GGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAG178730.12153342777849024No Hit
GCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCCCG177290.1205542517252198No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC173200.11777311974058363No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA171540.11664434734584132No Hit
TGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACA171530.11663754751213805No Hit
CCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTC170530.11595756414181134No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA167620.11397881253416066No Hit
CCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC164070.1115648715695009No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCC161940.11011650699070503No Hit
CCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGC161590.1098785128110907No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC158050.1074713716801342No Hit
GCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAG155480.10572381441839458No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT152290.10355466746705244No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG152280.10354786763334917No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT150900.10260949058229832No Hit
TGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCA150090.10205870405233369No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACTA168300.054.0617074
AGAAACT169400.053.624333
AAGAAAC171450.053.0254362
CTAACCA195250.046.5988738
CGCTCAG44850.042.513164
TCCCTGA137200.042.2198683
CTCCCTG143150.040.009212
GAGTCTA63350.039.4817772
ACATCGA37550.038.614484
AGGCGTA62050.038.556268
TAACCAG239650.038.510029
AACTAAC240750.038.1245656
ACTAACC241450.038.0126157
CTCGGCT62300.037.7038421
AAACTAA247400.037.1580245
TTCGTTC87550.036.517859
AGTCACA38400.036.247616145
CGATGAC12500.035.959595
AGTCTAA70000.035.731013
GAAGAAA134900.035.6849251
Produced by FastQC (version 0.11.5)