FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n01_MT_30905.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n01_MT_30905.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22666889
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT277120.12225762432594962TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATG191050.017.682468145
AACGTAT157550.013.3449266
GAACGTA166650.012.9640825
CGGGAAC162800.012.82517052
GGAACGT162600.011.9939584
CGATCTA27200.011.4612074
CCGGGAA179400.011.3959571
CGTATTC192200.011.3158758
ACGTATT191600.011.2378477
GAGCACC169300.010.790648145
CTCGTAT391050.010.678105145
TACTCGC73000.010.228651145
GGGAACG207450.010.0298293
TTACTCG794000.010.025034145
ACACGTA66150.09.6439905145
TATGCCG308750.09.626778145
CGTATGC376200.09.500186145
ATCTCGT445150.09.25009145
TCGTATG394800.09.199508145
GATCTCG512350.09.182944145
Produced by FastQC (version 0.11.5)