FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n01_MT_30904.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n01_MT_30904.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19612585
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT403730.20585251765639256No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT345610.17621848420287278No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA268710.1370089664366018No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA257480.13128305116332192No Hit
GGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGAT252570.12877955659593063No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC219000.11166299597936732No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG218820.11157121817445279No Hit
TGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACA204020.104025043103701No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATAC44500.036.1676149
GAGTCTA52050.030.3638692
AACAGAC53750.030.348915
CTCGGCT66000.029.7681031
TTGTACT48750.028.9991821
TAGCATA73500.028.8019818
GGCCAAC151000.028.134971
AGTCTAA56100.027.784143
AACCGAG159600.027.7553925
TGTACTC53800.027.085392
GAGCACC106050.026.112972145
GTTACGA64400.025.6674374
CGGATGT36800.025.6106911
CAATTAG45200.024.539993
GCCAGTA88400.024.5211183
AGTAAGT82300.024.311562145
TTACGAC68400.024.272725
AGTCACA65250.023.775896145
AAGAAAC160150.023.3584542
GTCTAAC68800.022.6560174
Produced by FastQC (version 0.11.5)