FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n01_MT_30903.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n01_MT_30903.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19725045
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA50350.023.6140214
CTATTAG102050.015.6289098
GATCTAT92950.013.9614085
AACGTAT147350.012.64475
CGGGAAC175850.012.6153121
GAACGTA166950.012.2892554
GGAACGT156250.012.109873
CTCGATG252950.012.037119145
TATGCCG243600.011.69562145
CCGGGAA150800.011.3937311
GGGAACG188750.011.0617392
GTATGCC276650.010.796289145
CTCGTAT332950.010.625485145
CGTATGC312600.010.575085145
TCGTATG343050.010.249254145
ACGTATT188600.010.1097576
TTACTCG686450.010.043372145
CGTATTC186050.09.9753757
GAGCACC173750.09.679906145
TATTAGT165300.09.5610089
Produced by FastQC (version 0.11.5)