FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n01_MT_30891.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n01_MT_30891.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19193619
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT476560.2482908512459271TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATG268400.011.776366145
CTCTAGT71200.010.8954756
ACTCTAG78500.010.8057865
GGGGAAT139250.010.3605891
GAGCACC156500.09.125554145
CTAGTTG83600.09.0188548
CGATCTA47150.08.764584
TCTAGTT99550.08.6662267
CGGGAAT104850.08.5047841
GGGAAAT181900.08.3299071
CGATCTC587750.08.214647145
CTCGTAT467600.08.154899145
TATGCCG417400.08.093584145
GCCGGAT108850.08.0590441
AAGGGGG138050.08.04709770-74
CGTATGC478150.07.990129145
GTATGCC445600.07.87422145
TACTCGC89850.07.826382145
CCGGGAT71000.07.76035931
ATCTCGT537650.07.7396193145
Produced by FastQC (version 0.11.5)