FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n01_MT_30890.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n01_MT_30890.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19416709
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT463730.23883038057582262TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCT110350.019.9058863
TGTACTC125750.018.4481682
CTCGATG326900.018.295565145
TACTCTA125900.017.9659964
TAGTTGT128850.017.329659
TACTCGC109100.017.010693145
TCTAGTT144050.016.3063377
TTGTACT140750.015.3490851
GAGCACC189650.014.143459145
ACACGTA62250.013.5090685145
CTCTAGT175500.013.2604826
ACATTAA147650.012.618463145
CTAACCA118350.012.3739348
AACTAAC139950.011.9665916
ACTAACC143200.011.1379267
TACGGAC111450.011.1235095
ACTCTAG219150.010.9830355
TATGCCG687650.010.964092145
GTATGCC740200.010.812516145
TGTCCTA91250.010.4873765
Produced by FastQC (version 0.11.5)