FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n01_MT_30887.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n01_MT_30887.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18046677
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAA148600.017.3193861
CGGGAAC164200.016.2920671
GAACGTA175150.015.3566464
AACGTAT173650.015.2390086
GGGAACG185850.014.7063673
ACGTATT185100.014.3350767
GGGTTAG94500.014.116033
GGAACGT188300.013.9761924
GGTTAGA123950.012.5754794
CGTATTC219600.012.4130838
TATTCAC221350.011.7580559
GTATTCA252700.010.7009949
TAGAACC152900.010.1468597
CGGAAAG167100.09.8485521
GAGCACC125150.09.8474045145
TGGGTTA134200.09.8320542
ACACGTA53650.09.728961145
AATCGGG311600.09.724842145
CTGGGTT220300.09.7080151
GAATCGG336850.09.684555145
Produced by FastQC (version 0.11.5)