FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n01_MT_30886.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n01_MT_30886.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20556794
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT505640.245972207533918TruSeq Adapter, Index 6 (97% over 36bp)
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT378400.18407539619261643No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA277860.13516699150655495No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC242900.1181604485602181No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT241440.11745022108019373TruSeq Adapter, Index 6 (97% over 36bp)
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT227310.11057658115365655No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT220590.10730758891683208No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT218640.10635899741953925No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCGT81600.025.8542254
AGGCGTA74000.024.5903918
CTAACCA174600.024.1658548
GTACTCT101950.023.3242633
CGTGCGT89800.022.927968
GCGTAAC97000.022.8701422
GAGAATG334800.022.670847145
CGCGTAA95550.022.6102751
TAGTTGT112550.021.7717089
ATCCCGA281250.021.2919864
TACTCTA121850.020.7648414
TGTACTC120900.020.4478322
TACGGAC216000.020.2727135
GAGCACC160350.020.118591145
GAAACTA220900.020.085584
TCGTGCG105250.020.0444077
TCACCCG154200.019.4172153
TCCCGAG308700.019.3986785
ACTAACC229050.019.0541067
GATCCCG320000.018.5547543
Produced by FastQC (version 0.11.5)