FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n01_MT_30849.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n01_MT_30849.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31924226
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT502490.15740084035240198TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGATC282450.014.6816062
TGATCCT237200.014.487534
GATCCTG251300.014.4247925
TTGATCC260550.013.7732343
AACGTAT412150.013.6501835
GGTTAGA170650.013.382434
CCGGGAA354650.013.0010871
CTGGCTC289250.012.5069999
CGGGAAC444300.012.4174051
TATTCAC454500.012.2026319
GGGAACG437500.011.9806222
GAACGTA509050.011.9347754
CCTGGCT266250.011.7086688
TCCTGGC277650.011.5673727
CAATTAG167700.011.369699
ATCCTGG321950.011.2144966
ACGTATT510900.011.0687256
GTATTCA518200.010.9545128
CGGAGTA149350.010.5823464
CGATCTA54500.010.3759724
Produced by FastQC (version 0.11.5)