FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n01_MT_30848.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n01_MT_30848.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21933430
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT333600.15209659410315668TruSeq Adapter, Index 2 (97% over 37bp)
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG219540.1000937837811961No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA90900.057.1043174
ATCTATT121950.044.7059946
TCGATCT124450.042.2919353
CTCGATC127750.040.631882
CTATTAG163150.037.6370968
TATTAGT231250.026.710239
GATCCTG222250.025.4107485
GATCTAT232900.023.937495
CTACTCT77600.023.4494049
CTGGCTC231850.023.2953369
GTGTCCT79600.022.1316094
TCTATTA277850.019.9344257
TCCTGGC281150.019.8293747
TGATCCT243900.019.290914
CATTATG181500.018.852516145
TGTCCTA97350.018.0963975
CGGGAAC454050.018.0104771
GGGAACG454050.017.9144732
CCTCGAT322350.017.857171
CCTACTC111400.017.8313888
Produced by FastQC (version 0.11.5)