FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n01_MT_30786.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n01_MT_30786.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18066432
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTATG165100.028.453697145
CTAACCA102450.017.6203218
CTCGGCT88500.017.4487341
GGCATAG197900.016.5585635
TGCCGTG88950.016.544775145
ATAGTTC203450.016.2848958
CGTGCGT83050.015.8875968
GCATAGT207050.015.86208256
ACTAGGC91450.015.8553685
AACGTAT188200.015.7943396
TAGTTCA208900.015.72108659
GCTCAGA119150.015.698415
ACACGTA52850.015.500447145
GAGCACC171100.014.95669145
GATCCTG109550.014.8901875
GAACGTA205900.014.7532515
GGCTCGT97050.014.5669254
CTAGGCA114450.014.4429636
CATAGTT237550.014.3440067
AGGCATA197500.014.3161544
Produced by FastQC (version 0.11.5)