FastQCFastQC Report
Wed 13 Mar 2019
H35K7BGXB_n01_MT_30785.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35K7BGXB_n01_MT_30785.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22518682
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG344200.010.320346145
GAATCGG438750.09.946915145
AATCGGG389750.09.467617145
GGGGAAT202400.09.2054941
CGGAGAA740350.08.979276145
ACACGTA65550.08.847617145
CCGGAGA803050.08.738599145
ACTCCGG914800.08.550753145
AGAATCG575300.08.543668145
CCGGGAT73100.08.5291461
TCCGGAG812700.08.42967145
CACTCCG971400.08.365975145
TCGGGGG257650.08.159751145
GAGCACC168150.08.062203145
CGGGAAC87550.07.9494961
CTCCGGA860100.07.939826145
CGGGAAT85400.07.89495331
CCGGGAA101050.07.89188671
GGGAAAT309550.07.7989811
CCCGAAT84500.07.7216531
Produced by FastQC (version 0.11.5)