FastQCFastQC Report
Thu 24 Feb 2022
H35CCBGXL_n01_FebB3_23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35CCBGXL_n01_FebB3_23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4027340
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCGCTATGTTCATCGGGAGCGAGATCGGAAGAGCACACGTCTGAACT405751.0074888139566065Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATAAGTATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAACTGCC59380.1474422323419428No Hit
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC51950.12899333058544846No Hit
AGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAAGCATAGA50740.1259888661002051No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)