FastQCFastQC Report
Thu 24 Feb 2022
H35CCBGXL_n01_FebB2_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH35CCBGXL_n01_FebB2_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6407179
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAGTATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAACTGCC187820.29313992944476813No Hit
TAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAACTGCCTG132220.20636226957292747No Hit
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC132000.206018904731708No Hit
GTTGAATATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGA112140.17502242406525556No Hit
GGAGATTCCCAAATAGGTCAACCTTTTGAACTGCCTGCTGAATCCATGAG97210.15172043734067675No Hit
TTCCCATATAGGTCAACCTTTTGAACTGCCTGCTGAATCCATGAGCAGGC72010.11238955552825979No Hit
AGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAAGCATAGA65990.1029938448730713No Hit
TTGAAATATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGA65470.10218225524837063No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)