FastQCFastQC Report
Tue 12 Mar 2019
H357TBGXB_n02_Antennae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH357TBGXB_n02_Antennae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549717546
Sequences flagged as poor quality0
Sequence length91
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33699710.613036826734288No Hit
GGCTGGAATACCTCGACGTTATTCAGATTACCCAGATGCTTACACAACAT19813400.36042873552375204No Hit
GTTTTACCTATAAACTCACAAATTCGAATTTTAGTAACAGCTGCTGATGT17329420.3152422571572784No Hit
GCATTAGTTTTATCAATTGCAATTTTAATAATCCTTCCTTTTTATAATTT11410230.20756532301044653No Hit
GTTACTTTAGGGATAACAGCGTAATTTTTTTGGAGAGTTCATATCGATAA10767900.19588059501378913No Hit
GGAGTTAATTTAACATTTTTTCCTCAACATTTTTTAGGATTGGCTGGAAT10513410.19125112662858318No Hit
GAAACTATTAGAAATATTATTCGACCTGGAACATTAGCTGTTCGATTAAC10192690.18541685769658878No Hit
GAATTTTAGTAACAGCTGCTGATGTTATTCATTCTTGAACAGTACCTGCT9837060.17894753535845845No Hit
GCTCCATTTACTATTGCAGACTCAATTTATGGATCAACATTTTTTATAGC9516880.17312308965302703No Hit
GCAGACTCAATTTATGGATCAACATTTTTTATAGCAACAGGATTCCACGG9441060.1717438358789443No Hit
GGATTGGCTGGAATACCTCGACGTTATTCAGATTACCCAGATGCTTACAC9293580.16906100355763432No Hit
GTTTTATCAATTGCAATTTTAATAATCCTTCCTTTTTATAATTTAAGAAA8785270.1598142548646246No Hit
GCTGGAATACCTCGACGTTATTCAGATTACCCAGATGCTTACACAACATG8394080.15269805486616211No Hit
CCTCGACGTTATTCAGATTACCCAGATGCTTACACAACATGAAATATTGT8355650.1519989685757638No Hit
GGAATACCTCGACGTTATTCAGATTACCCAGATGCTTACACAACATGAAA7819550.14224668753796701No Hit
GTTAATTTAACATTTTTTCCTCAACATTTTTTAGGATTGGCTGGAATACC7801010.14190942342597157No Hit
GGAGAGTTCATATCGATAAAAAAGATTGCGACCTCGATGTTGGATTAAGA7441580.13537097467869436No Hit
GCTGATGTTATTCATTCTTGAACAGTACCTGCTTTAGGAGTAAAAGTTGA7412580.13484343102994206No Hit
GTTCGATTAACTGCTAATATAATTGCTGGACATTTATTATTAACTCTTTT7409970.13479595210155434No Hit
GATGTTATTCATTCTTGAACAGTACCTGCTTTAGGAGTAAAAGTTGACGG7343240.133582055974615No Hit
GATTACCCAGATGCTTACACAACATGAAATATTGTATCAACTATTGGATC7217440.13129360800864812No Hit
CATTCTTGAACAGTACCTGCTTTAGGAGTAAAAGTTGACGGTACACCTGG7070300.12861696068184078No Hit
CAAGGAACACCCGCTATTCTTATACCTTTTATAGTATGTATTGAAACTAT6951480.1264554870147805No Hit
GGATTAACGTTAAATAATAAATGATTAAAAAGTCATTTCATTATTATATT6931410.12609039042752332No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG6873860.12504348915215452No Hit
GTTTTACTAGGAATCTATTTTACAATTCTTCAAGCTTATGAATATATTGA6800480.12370862180920818No Hit
GTAACAGCTGCTGATGTTATTCATTCTTGAACAGTACCTGCTTTAGGAGT6778800.12331423745386508No Hit
AAAAAATTAAGTTACTTTAGGGATAACAGCGTAATTTTTTTGGAGAGTTC6769960.12315342759679714No Hit
ACCCAGATGCTTACACAACATGAAATATTGTATCAACTATTGGATCAACT6711690.12209342868601104No Hit
GGATCAACTATTTCATTATTAGGAATTTTATTCTTTTTTTTTATTATTTG6638770.12076692927680355No Hit
AACATTTTTTATAGCAACAGGATTCCACGGAATTCATGTATTAATCGGAA6601930.12009676693128511No Hit
AATTTAACATTTTTTCCTCAACATTTTTTAGGATTGGCTGGAATACCTCG6583500.11976150384692286No Hit
GATCAACATTTTTTATAGCAACAGGATTCCACGGAATTCATGTATTAATC6569610.11950882863033083No Hit
GTTCATATCGATAAAAAAGATTGCGACCTCGATGTTGGATTAAGATATAA6539940.1189690969041763No Hit
CAACATTTTTTAGGATTGGCTGGAATACCTCGACGTTATTCAGATTACCC6508700.11840080505634797No Hit
GCAATTTTAATAATCCTTCCTTTTTATAATTTAAGAAAATTCCGAGGGAT6291400.1144478659227661No Hit
GCTGCTGATGTTATTCATTCTTGAACAGTACCTGCTTTAGGAGTAAAAGT6154620.11195967901668541No Hit
CGAATTTTAGTAACAGCTGCTGATGTTATTCATTCTTGAACAGTACCTGC6055210.11015129577108315No Hit
AGAAATATTATTCGACCTGGAACATTAGCTGTTCGATTAACTGCTAATAT6047250.11000649413508079No Hit
ATTCAGATTACCCAGATGCTTACACAACATGAAATATTGTATCAACTATT5972930.10865452710145075No Hit
AAAAAGTCATTTCATTATTATATTTATTGGAGTTAATTTAACATTTTTTC5940160.10805840277836065No Hit
AAAAAGATTGCGACCTCGATGTTGGATTAAGATATAATTTTGGGTGTAGC5907920.1074719197702305No Hit
CGACCTGGAACATTAGCTGTTCGATTAACTGCTAATATAATTGCTGGACA5905230.10742298554901866No Hit
AGTTTTACCTATAAACTCACAAATTCGAATTTTAGTAACAGCTGCTGATG5885840.10707025895076669No Hit
GATTATTAGATGTTGATAACCGAGTAGTTTTACCTATAAACTCACAAATT5610990.102070418541816No Hit
GGGATAACAGCGTAATTTTTTTGGAGAGTTCATATCGATAAAAAAGATTG5591360.10171332606509162No Hit
GAAATATTATTCGACCTGGAACATTAGCTGTTCGATTAACTGCTAATATA5574900.10141389956652394No Hit
CAATTTATGGATCAACATTTTTTATAGCAACAGGATTCCACGGAATTCAT5555250.10105644326659351No Hit
GTTCCTCAAGGAACACCCGCTATTCTTATACCTTTTATAGTATGTATTGA5536260.10071099313246223No Hit
GTTATTGCATTAGTTTTATCAATTGCAATTTTAATAATCCTTCCTTTTTA5498810.1000297341791597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT30463500.079.566311
AGCAGTG30995300.077.885452
GCAGTGG34141500.070.68833
CAGTGGT34211900.070.3401264
AGTGGTA34816550.069.2255555
GTGGTAT35720000.067.357826
GGTATCA35649400.067.357448
TGGTATC35803650.067.047667
GTATCAA67368150.035.9350179
CAACGCA35476400.035.5291312-13
GCAGAGT35749200.035.52003516-17
AGAGTAC35830900.035.49043718-19
CATGGGG16107450.035.3516824-25
ATCAACG35822000.035.22072610-11
TACATGG35741000.035.1877522-23
ACGCAGA36034000.035.02153814-15
CATGGGA12991000.034.7111924-25
AGTACAT36509950.034.64208620-21
CGCAGAG35694850.033.83348516-17
AACGCAG35680100.033.51172614-15
Produced by FastQC (version 0.11.5)