FastQCFastQC Report
Tue 12 Mar 2019
H357TBGXB_n01_Antennae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH357TBGXB_n01_Antennae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549717546
Sequences flagged as poor quality0
Sequence length28
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGG9478650.17242764159468907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG2442200.019.3553541
GACTAAC1445000.019.1339191
GTGCGAG1240700.019.0403613
GGGACCT1479200.018.9712411
CGTGAGC1347850.018.8794171
GACGACG1031550.018.8718419
TTAGGCA2602250.018.5695921
AAACCGG1260750.018.3723952
ACGAGGA1173900.018.353031
TGTAAAC1693350.018.3225827
ACTGATG1470200.018.2730451
GACCTGG1427250.018.267821
AGCTTGA2020800.018.2064971
GTGCTGC1499550.018.1270569
CCTACCA1550550.018.12311
AAGGCAG2092550.018.0996761
ACGCCGA1851350.018.0772951
TTGAACG1361000.018.0550381
GTAACGG1560950.018.03486310
CAATGGA1237100.018.0025798
Produced by FastQC (version 0.11.5)