FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40904813
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1064237426.01741266975111No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG487070.11907400725679886No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA401800.068.842121
GGTAGTA390700.068.795584
AGTAGGT295750.068.777687
TAGTAGG294500.068.714596
GAGGTAG414350.068.578032
TAGGTTG294950.068.5277949
GTAGTAG391750.068.41695
TGTATAG271750.068.3918914
TATAGTT248600.068.3028716
TTGTATA272800.068.1796413
GTTGTAT224150.068.0695612
AGACCCT261550.067.899527
TGAGACC260200.067.8843155
AGGTAGT395800.067.750773
TCCCTGA257400.067.748781
GTATAGT273750.067.67332515
TAGTTAA246850.067.62591618
GAGACCC263700.067.265326
ACTTGTG226450.067.1724615
ATAGTTA248700.067.0392817
Produced by FastQC (version 0.11.5)