FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5123970
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG419590.8188767693799925No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA330040.6441099381924563No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT233260.45523295413517256No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA212430.4145808816210868No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC191710.374143486398242No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT150940.29457627581738377No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG134070.261652585787973No Hit
GATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACC129370.25248001061676784No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG117050.22843615399777908No Hit
TTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCAAT108920.2125695505633327No Hit
CGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCAC102100.19925955850639251No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC83540.16303764463882497No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGC77420.15109378079887276No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCA76870.1500203943426679No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGG73740.14391184960099299No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC70700.13797894991578796No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACTG65470.12777202052314904No Hit
GAGCAGCTCCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGG61610.12023879921232951No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCAA55050.1074362262074134No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTGT66200.069.2918412
AGTAGGT225500.069.159017
GTAGGTT224100.069.154558
GGTAGTA304200.069.152864
TAGTAGG224150.069.130296
TAGGTTG224050.069.106949
AGGTTGT237700.069.0773310
TGAGGTA315800.068.930071
GTTGTAT173400.068.8851112
TATAGTT189950.068.80559516
GGTTGTG66850.068.7226111
GTGTGGT65850.068.652115
GAGGTAG323900.068.60362
GTAGTAG306000.068.540565
TGTGGTT59550.068.4643416
TTGTATA216300.068.40053613
TGTATAG216350.068.3692514
CTGAGAC203750.068.298874
TGAGACC203500.068.193915
GGATAGG26850.068.0493914
Produced by FastQC (version 0.11.5)