FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5525389
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG327870.5933880854361566No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA296350.5363423281148169No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT246410.44595955144515614No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC201920.36544033370320167No Hit
TTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCAAT181600.32866464243512994No Hit
GATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACC180000.3257689187132345No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA162610.2942960215108837No Hit
CGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCAC151780.27469559156830403No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG138770.25114973805464197No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT120770.21857284618331851No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGC104440.1890183659467234No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCA103280.18691896624834922No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG91000.1646942866828019No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGG87830.1589571340587966No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC84160.15231506777169898No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACTG83600.15130156446903556No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCAA82060.14851443038671122No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC75160.13602662183603725No Hit
CGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACTGT68110.12326733918643555No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT202850.069.182518
AGTAGGT204850.069.0169457
AGGTTGT214500.068.9934210
GGTAGTA280250.068.9773254
GTTGTAT154800.068.91875512
TGAGGTA288050.068.892511
TAGTAGG204200.068.865156
GGTTGTG60850.068.8563711
TAGGTTG203800.068.825749
GTTGTGT60850.068.6260312
TATAGTT171800.068.5204416
GAGGTAG295700.068.516742
GTGTGGT60300.068.2612415
GTAGTAG283200.068.136035
GTATAGT193750.068.1163715
TTGTATA194700.067.9832713
GCTACAT61500.067.9556962
CAGCACG58200.067.90624
CTAACTT156000.067.895812
CTGAGAC178900.067.8561554
Produced by FastQC (version 0.11.5)