FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5014379
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG529111.0551854975461568No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA234630.46791437184943535No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA206300.4114168474301604No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT166290.3316263090604041No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT159730.3185439313621886No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG137430.2740718242478281No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC135280.26978415472783374No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC130560.2603712244327762No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG126610.2524938781053446No Hit
GATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACC118370.2360611353868545No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC108390.21615837175450842No Hit
CGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCAC98820.19707325672830076No Hit
TTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCAAT98490.19641514931360393No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGC73430.14643887109450643No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGG65840.13130240055647968No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCA64200.12803180613192583No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACTG51910.10352229059670201No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT279350.069.355328
AGGTTGT297150.069.2242410
TAGGTTG279600.069.180159
GGTAGTA384350.069.17644
AGTAGGT281650.069.174917
GTTGTAT214800.069.11819512
TAGTAGG280950.069.090916
GGTTGTG84050.069.051311
TGAGGTA398850.069.026651
GTTGTGT83950.068.96706412
GAGGTAG407500.068.92722
TATAGTT239050.068.8881116
GTAGTAG385750.068.762285
GCTACAT80200.068.7547762
TTGTATA272300.068.7393313
TGTATAG272750.068.728414
GTGTGGT82950.068.702615
AGACCCT255900.068.571247
CTGAGAC256650.068.564884
TGAGACC256800.068.347935
Produced by FastQC (version 0.11.5)