FastQCFastQC Report
Fri 1 Mar 2019
H3537BGXB_n01_HFL37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3537BGXB_n01_HFL37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5016279
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG285370.5688878150517546No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA277700.5535975969438701No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT227100.45272601464153006No Hit
TTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCAAT179100.35703755712152374No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC178330.3555025547821403No Hit
GATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACC157930.3148349603361376No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG138050.27520399084660163No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA136590.2722934669303681No Hit
CGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCAC133100.265336118664851No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC115370.22999119466839862No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC107310.21392350784316425No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCA103870.20706583505423043No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT101370.20208206122506342No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACTG88180.17578767050237834No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCAA86840.17311636772994485No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGC84820.1690894784759779No Hit
CGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACTGT76810.15312146712732685No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGG72920.145366715049143No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG71110.14175846279682608No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACT53800.1072508128036738No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT195500.068.9745948
GGTAGTA263900.068.9501654
TGAGGTA271400.068.9081
AGGTTGT206400.068.8209510
AGTAGGT197400.068.7879647
TAGGTTG196000.068.7814949
GTTGTAT148200.068.70792412
TAGTAGG197000.068.6145256
GAGGTAG277950.068.58282
GCTACAT67650.068.443972
TATAGTT163200.068.38805416
TGCTGGG59150.068.2931613
GTTGTGT59900.068.2534312
GTAGTAG265600.068.220075
CTGAGAC175050.068.193484
AGCTTAT36450.068.164652
GGTTGTG59950.068.0799311
CTAACTT153650.068.06747412
TGAGACC175400.067.9186255
TTGTATA184250.067.91582513
Produced by FastQC (version 0.11.5)